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C Perricone, C De Carolis, R Glutathione: a key player in autoimmunity

Gooding J, 2021, 'Ultrafast generation of highly crystalline graphene quantum dots from graphite paper via laser writing', Journal of Colloid and Interface Science , 594, pp

292 Reduced membrane-associated RING finger protein 2 (March 2) disrupts its interaction with PKM2, altering glucose metabolism and increasing lactate production, which enhances H3K18la and p53-mediated apoptosis in VSMCs, worsening aortic aneurysm/dissection

The reporter ion intensities were defined as intensities multiplied by injection time (to obtain the total signal) for each isobaric labeling channel summed over all MS/MS spectra matching to the protein group as previously validated (Tyanova et al., 2016)

In particular, multiplexed edited detection allows for GSH editing simultaneously with others metabolites in a single acquisition

Then, membranes were incubated for 2 hours using primary antibodies for CYP 11A1, 21A2, and CYP 19 using the dilution of 1:1000 and then washed twice using Tween-TBS (0.2 ml Tween/ liter TBS) for 20 min, then with TBS for 15 min