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After washing with PBS-T, the membrane was incubated with the ECL prime western blotting detection reagent (GE Healthcare UK, Little Chalfont, England) and scanned using a ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) or ImageQuant LAS 500 (GE Healthcare UK)

Abstract Simple Summary Malignant peripheral nerve sheath tumor (MPNST) is a soft tissue sarcoma with limited therapeutic interventions and a poor prognosis

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[2] Data discrepancy of around 1-3cm exists because of manual measuring