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To this end, we tested the effect of selective metabolic inhibitors UK5099 (mitochondrial pyruvate carrier inhibitor) [39] V-9302 (inhibitor of the glutamine transporter ASCT2) [40, 41] V-9302 reversed the competitive outgrowth of MEC-1/N M cells, resulting in a relative enrichment of MEC-1/N WT cells over time (Fig

Lifespan is how long an organism lives

Academic Press: Oxford, 2019

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Atanasov AG, Waltenberger B, Pferschy-Wenzig E-M, et al

Therefore, we preformed DCFH-DA and C11-BODIPY staining followed by flow cytometry and found that WT ACTL6A reversed the increase in ROS levels (in cells treated with or without H 2 O 2 or both H 2 O 2 and NAC) and lipid peroxidation levels (in cells treated with or without erastin or both erastin and Fer-1), while ACTL6A HR-deleted mutant did not reverse these high levels (Fig