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CHAC2 itself is regulated both transcriptionally and post-translationally, with its degradation mediated by the ubiquitin-proteasome pathway ( In lung cancer, however, the CHAC2-mediated increase in ROS triggers the MAPK pathway

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The cyclin-dependent kinase inhibitor p21 is induced by p53 in response to oxidative stress and competes with Keap1 for the binding to the DLG motif of Nrf2, thus compromising Nrf2 ubiquitylation and promoting the Nrf2-dependent antioxidant response (20, 34)

The resultant metabolite extracts from CAR T cells and BM were recovered by centrifuging at 13,226 g at 4 C for 15 min

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