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Briefly, hepatic organoids were lysed in T-PER TM buffer (Thermo Fisher Scientific), and lysates were collected by centrifugation at 8000x g for 10 min

MICC stands for Methionine, Inositol, Choline, and Cyanocobalamin (B12), a combination of lipotropic compounds that promote fat metabolism and support liver health

This ECM-independent system combined with small molecules can produce 300-500 organoids per ml of culture media, routinely producing tens to hundreds of thousands of organoids (see Supplementary Fig