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A broad methodological and mechanistic analysis was provided in a critical review discussing thrombin versus factor Xa cleavage: this review emphasizes both the specificity of thrombin at the consensus site and the risk of off-target cleavage in the fusion partner (target protein or POI), urging careful characterization of the final product [150]

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Effect of New Zealand blackcurrant extract on performance during the running based anaerobic sprint test in trained youth and recreationally active male football players

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For production yield experiments, NIP IgG1 WT and REW were produced in Expi293 cells using the Expifectamine Transfection Kit (Thermo Fisher) and purified as above