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TMRE labeling To assess the impact of Cu on mitochondria, control, and treated cells were incubated with 250 nM of the mitochondrial membrane potential dye TMRE (Thermo Fisher Scientific) for 30 min, and then images were examined under a Zeiss LSM 700 confocal microscope

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The dorsolateral SN Nigrosome 1 hyperintense vanished in the QSM and SWI of PD patients, commonly referred to as the swallow-tail sign disappearance

Recent innovations in SFM have improved ethical compliance, GMP compatibility, and positioned these systems as key future directions in mammalian cell culture (Even et al., 2006)

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