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Also, free radicals can damage sperm DNA through oxidation of purine and pyrimidine bases, breakage of DNA single and double strands, protamine deficiency, chromosomal rearrangement and gene mutation and the detrimental consequences are cell damage, cell apoptosis, and impaired fetus development

Ensure no air bubbles are present in the syringe

Fifteen healthy cats, 61 cats with CKD, and six cats with acute-on-chronic kidney disease (ACKD) were included in the study

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After deparaffinization and rehydration, all sections were treated with microwaves in 10 mmol/L citrate buffer (pH 6.0) for 10 min for antigen retrieval