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Elevated insulin levels directly suppress AOD-9604's lipolytic activity

Non-specific binding sites were blocked by incubating the sections in 10% normal goat serum in PBS, 1 h at RT

However, excessive activity of NNMT can lead to issues such as metabolic irregularities and increased cellular stress

9 This is a crucial distinction to understand: GRAS is a food-law safety concept, self-affirmable by a sponsor, that addresses ingredient safety at defined exposure levels