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2.11 Determination of related enzyme gene expression A MagMAX 96 Total RNA Isolation Kit (Takara Biomedical Technology Co., Ltd., Kyoto, Japan) was used to extract RNA from samples at each sampling point

Once reconstituted for in-vitro use, refrigerate and use within 3045 days

377 In AD mice, injection of BM-MSC-EVs reduced plaque deposition and brain A levels, decreased the activation of astrocytes and microglia, downregulated pro-inflammatory cytokines (TNF- and IL-1), and upregulated anti-inflammatory cytokines (IL-4 and IL-10)

10.1111/cns.12834 16 CerveriG.GesiC.MencacciC

catecholamines, growth hormone, and cortisol), which are released in response to increasing metabolic demands during exercise [64,65]