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Purification of the NTD for crystallization and assays Cells were thawed in lysis buffer (600 mM NaCl, 100 mM TrisHCl, pH 7.5) supplemented with 1.2 mM PMSF, while gently stirring for 30 min

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CeruttiRPirinenELampertiCMarchetSSauveAALiWet alNAD+-dependent activation of Sirt1 corrects the phenotype in a mouse model of mitochondrial disease

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they are usually converted into ester or anhydride derivatives

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