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For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)
Vitamin B6 works with the other B vitamins to build bone and promote healthy mental function

92:1061-1085 [PMC free article] [PubMed] 67

Intended for in vitro research applications only
This process was fundamental in order to buffer ROS flux and a consequent detrimental over increase of self-digestion (Desideri et al., 2014
Int J Mol Sci 18(5):1092 Sachdeva MM, Cano M, Handa JT (2014) Nrf2 signaling is impaired in the aging RPE given an oxidative insult
