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After staining, cells were fixed in 4% PFA, dried onto glass slides and examined by epifluorescence microscopy (Olympus, BX60)

Dysregulation of this same pathway has also been shown to occur via mutations, deletions, or suppression of PTEN (20-40% of IDH wild-type GBM)
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61 The sensor protein recognizes specific danger signals or pathogen-associated molecular patterns and undergoes conformational changes upon activation
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Mutations in a second amino acid transporter that contains a heavy and light chain and is encoded by two genes (SLC7A9 and SLC7A3) was identified in Type II and III patients [34]

PD and HC cohorts found increased serum serine in PD patients compared to controls 25 , which is in line with our work
