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All primary antibody incubations (1:1000 in TBST, overnight at 4 C) were followed by incubation with a secondary HRP-conjugated antibody (1:5000 in TBST, 1 h at room temperature), and the signals were visualized using a chemiluminescent HRP substrate on a chemiluminescence imaging system (CHAMPCHEMI TM Chemiluminescent Imaging and Analysis System)

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The main component of this slimy goo is a substance called polyglutamic acid, or more specifically poly-gamma-glutamic acid or Gamma PGA (-PGA)

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