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10.1186/s12943-020-01258-7 Summary Keywords ulcerative colitis, GHK-Cu, SIRT1/STAT3 pathway, co-culture system, network pharmacology, molecular docking Citation Mao S, Huang J, Li J, Sun F, Zhang Q, Cheng Q, Zeng W, Lei D, Wang S and Yao J (2025) Exploring the beneficial effects of GHK-Cu on an experimental model of colitis and the underlying mechanisms

The catalytic mechanism of two sites involved in GSTs is as follows: (1) GSH binds to the G site to form the strong nucleophilic thiolate anion, (2) the electrophilic substrate bound to the H site reacts with the thiolate anion of GSH to form the GSH conjugate, which will be released via the C-terminus [34]

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6 Phase I reactions are frequently mediated by cytochrome P450 enzymes, such as CYP2E1 in the case of acetaminophen

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