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10.1186/1477-3155-10-16 207 XuM.HalimuG.ZhangQ.SongY.FuX.LiY.et al (2019)

Two-dimensional gel electrophoresis The extracted histone proteins were dissolved in 150 l rehydration buffer (8 M urea, 2% CHAPS, 0.5% IPG buffer and 0.002% bromophenol blue), and then loaded onto isoelectric focusing (IEF) strips using the following programme: 20 V for 10 h (rehydration)

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Sun et al devised the combination strategy for the nose-to-brain delivery of the neuroprotective agent paeonol, which is challenged by poor water solubility and rapid metabolism, using an SLN-combined ion-sensitive ISG

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