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For specific analysis of cytoplasmic and nuclear proteins, the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit was employed for TM3 cells

Vitamin B12, aka cobalamin, is an essential nutrient that plays an important role in overall health

Non-PDZ scaffolding proteins: cingulin and paracingulin Cingulin and paracingulin homodimers consist of a coiled rod and globular head, which have a shared ZO-1-Interaction-Motif (ZIM) at the N-terminus

Unlike scleroderma, the disease primarily involved the extremities but spared the face

The symptoms often appear in the hands and feet

Fukutomi T, Takagi K, Mizushima T, Ohuchi N, Yamamoto M