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Speicher D.W, Marchesi V.T

J Immunol 165(3):12201227

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Experimental evidence points a multifactorial etiology, linking hyperglycemia with the activation of multiple cell phenomena such as polyol pathway, advanced glycation end-products formation, protein kinase C (PKC) and nuclear factor K signaling, among many others (Pacher, 2008

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To test LaccID in a similar manner, we created an ER-targeted construct by appending a KDEL ER retention sequence and prepared analogous constructs for APEX2 and HRP for comparison