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Several studies have made use of pimonidazole injections, to demonstrate an increase in hypoxic regions in primary tumors following anti-angiogenic treatment (86, 89, 115)

Lpez M, Alvarez CV, Nogueiras R, Diguez C (2013) Energy balance regulation by thyroid hormones at central level

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This approach enables researchers to track cumulative effects and optimize protocols based on progressive findings
