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cDNAs were reverse transcribed in the presence of a mixture of oligo dT and random primers (9:1) at 50C for 50 min using ThermoScript Reverse Transcriptase or SuperScript IV Reverse Transcriptase (Invitrogen) following the manufacturers instructions

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doi: 10.1186/1475-2891-11-79

I had tachycardia starting 30 hours after Moderna shot 1 and its been persisting since then, Im overdue for dose 2, Did you end up taking the 2nd shot