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[DOI] [PubMed] [Google Scholar] 342.Harvey CJ, Thimmulappa RK, Singh A, Blake DJ, Ling G, Wakabayashi N, et al

Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis

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Immunofluorescence (IF) staining Panc-1, MIA PaCa-2 and BxPC-3 cells were cultivated in 24-well plates after treatment, fixed with 4% paraformaldehyde for 10 min, washed with PBS three times, treated with permeabilization solution (1% Triton X-100 in PBS), washed with PBS again, and blocked with 5% bovine serum albumin (Sigma-Aldrich, Germany) for 1 h