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After the reaction, the optical density (OD) was measured at 450 nm by a microplate reader (Roche Molecular Diagnostics, Light Cycler 480II, Carlsbad, CA, United States)

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Without formal reproductive toxicity studies following OECD Test Guideline 414 (prenatal developmental toxicity) and 416 (two-generation reproduction toxicity), we are operating without the data that regulatory science requires before concluding a compound is safe for use during conception attempts or pregnancy