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-galactosidase fused to the HIV-1 TAT protein transduction domain (GRKKRRQRRRPQ) was grown in Escherichia coli (BL21) and expressed with 100 mM IPTG for 48 h at 37 C

The Reconstitution Protocol: A Step-by-Step Guide Alright, you've got your supplies, you understand your compounds, and your workspace is clean

Intracellular lipid levels additionally influence CMA activity

10.1073/pnas.97.2.559

Exp Cell Res 339: 9095, 2015
