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Compared to strong oxidants like the hydroxyl radical, 4-HNE and MDA are relatively stable and able react with macromolecules, such as proteins distal to the site of origin

doi: 10.1146/annurev-cellbio-100913-013325 154 ItohN.ItohY.TassoniA.RenE.KaitoM.OhnoA.et al

The cells were incubated in 6-well plates (2 mL/well) at a density of 150,000 per well for 24 h, followed by treatment with test compounds with or without cisplatin (1 M) for 48 h

10.1203/PDR.0b013e318212f16b 14 FryeR

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Paraffin slices were deparaffinated in xylene, rehydrated in graded ethanol and stained with haematoxylin and eosin