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Plaque-forming assays HSV-1 (MOI = 0.1) was first co-cultured with different concentrations of WL-1 (0, 2, 10, and 50 M) or acyclovir (50 M) for 2 h and then diluted to infect Vero cells

After 180 days of exposure, the lowest observable effective concentrations (LOECs) at which citalopram significantly reduced the mean spawning of adult fish were 10 and 1 g/L, respectively, whereas the fertilization rate was not significantly altered

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