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We have determined the optimal emission wavelengths ( em ) for each OPAamino acid derivative for fluorometric detection: aspartate: 450 nm, glutamate 452 nm, serine 448 nm, glutamine 450 nm, histidine 448 nm, glycine 448 nm, threonine 449 nm, arginine 454 nm, taurine 448 nm, alanine 446 nm, tyrosine 450 nm, GABA: 448 nm, methionine 448 nm, tryptophan 445 nm, valine 453 nm, phenylalanine 451 nm, isoleucine 448 nm and leucine 445 nm