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This review synthesizes current evidence on CoQ10s mechanisms in safeguarding female fertility, including its effects on oocyte maturation, embryonic development, and ovarian aging

Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

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Although beyond the primary scope of this review, it is worth mentioning that emerging evidence highlights the gut microbiome as a potential mediator of circadian disruption-associated breast carcinogenesis, with studies indicating that NSW can alter microbial composition and diurnal rhythmicity, thereby modulating systemic inflammation, estrogen metabolism, and immune function, all of which are relevant to BC risk 260

10.1016/j.jinf.2021.05.010 21 DixonS