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Thus, a GST 4QN fusion tag is recommended to prevent aberrant pulldown experiments

Fumarate itself is a Michael acceptor, and the covalent attachment to protein cysteines corresponds to the post-translational modification known as succination [142] and fumarate has been recently demonstrated to yield a glutathione thioether in in vitro treated cells [142]
Diabetes 2011;60:27109

PMID: 27708183

[DOI] [PMC free article] [PubMed] [Google Scholar] 62.Murphy S., Rahmy S., Gan D., Liu G., Zhu Y., Manyak M., Duong L., He J., Schofield J.H., Schafer Z.T., et al

Additional in vitro studies indicate the formation of hydroxylated derivatives of STG, as well as N -acetyl-cysteine adducts of formed hydroxyquinones in humans (Pfeiffer et al
