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Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich

showed that the serum antibody targeting LAM in BCG-vaccinated NHP presented limited NK cell activation, while the antibody targeting LAM in lavage fluid showed activation of NK cells (59, 61)

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For both the control and the rd1 case, the confidence intervals reveal notable level of uncertainty in these parameters

62 Topical minoxidil has demonstrated efficacy and safety, with rare and typically mild side effects, including local irritation, hypertrichosis, and headache