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Coverslips were washed 3 times in PBS, fixed in 4% PFA for 10 min, stained with DAPI for another 5 min, washed in PBS, and mounted in a drop of Prolong Gold (P36934, Thermo Fisher Scientific)
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Additionally, this approach can help identify optimum combination drug regimens
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As we all know, Rb affects tumor progression by regulating apoptosis (Li et al., 2019
