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Then 80% of the supernatant was transferred to 0.2 m centrifugal filter tubes and samples were centrifuged again at 10,500 RPM at 4C for 5 min

[DOI] [PMC free article] [PubMed] [Google Scholar] 128.Vilks K, Videja M, Makrecka-Kuka M, Katkevics M, Sevostjanovs E, Grandane A, et al

Along with mitochondria, synapses are considered end effectors of many molecular mechanisms related to ASD, and the convergence of different neurodevelopmental pathological mechanisms on synapses can at least partially explain the behavioral similarities in different individuals on the spectrum