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Volpe, M

Mice were anesthetized with isoflurane for the duration of the isolation

Sections were then incubated with rabbit polyclonal anti-GFAP antibody abcam, ab7260, Cambridge, UK) (1:200 dilution) overnight at 4 C, followed by incubation with biotinylated goat anti-rabbit IgG secondary antibody (abcam, ab6720, Cambridge, UK) (1:400 dilution) for 30 min at room temperature

Reveals pathway regulation under different dietary or environmental conditions

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