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2025;16(1):e13677
Validation of a GC-MS and HPLC-ELSD method to study intestinal permeability

The solution was incubated with 0.5 mL pre-equilibrated Ni-NTA resin and rotated at 4 C for 1.5 h to remove His-tagged 3C protease and BirA

In human research of chronic insomniacs DSIP had normalizing influence on sleep regulation

This protein is secreted by neutrophils, monocytes/macrophages, and other cell types (such as epithelium) during pathogen infections, but it is also present in the intracellular milieu (Zhou et al., 2023)

After 4 to 6 days, the dose of SA was gradually reduced to a holding dose of 10 to 30 g per day
