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A challenge with this method is that the active hydrogen on NADPH can undergo enzyme-catalysed exchange with water, which leads to loss of labelling, but it is feasible to account for such losses by measuring the exchange rate between NADPH and water 60

In the past, this was not considered to be the case as Pkd1 and Pkd2 mutant kidneys do not form cysts when cultured ex vivo

doi: 10.3390/cells12071012 56 ItoM.HirayamaM.YamaiK.GotoS.ItoM.IchiharaM.et al

In oral form, phosphatidylcholine has to survive digestion and is partially broken down before it reaches the bloodstream

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in relation to temperature and feed composition
