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Wnt4 signaling is known to play a key role in nephrogenesis, as its activation promotes renal fibrosis in mouse models (Kispert et al., 1998

J.PengX.et al (2012)

It also helps maintain balance between the body and the environment

Single- or double-antigen labeling of formalin-fixed and paraffin-embedded 4-m-thick sections by immunocytochemistry (ICC) or immunohistochemistry (IHC) included the following steps: deparaffinization, rehydratation, boiling in EDTA buffer (1 mM EDTA, 0.05% Tween 20, pH 8.0) for 15 min, 1% H 2 O 2 incubation for 15 min and blocking in SuperBlock solution (ScyTek Laboratories, Logan, UT) for 30 min, followed by primary antibody application

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Due to the different cellular mechanisms that characterize the different MS stages, modeling the disease as a whole is not feasible with one given model