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To minimize potential confounding, we considered pre-specified covariates based on biologic interest, risk factors for ASCVD, or associations with TMAO or ASCVD

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1025 l of the heated sample (in two replicates) and 200 l of Infinity Triglyceride Reagent (ThermoFisher, TR22421) were added to separate wells of a 96-well plate and incubated at 37 C for 30 min