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Ice-crystal formation during each freeze-thaw cycle inflicts mechanical damage on molecular structure, and pre-splitting eliminates the cumulative loss that comes from thawing one vial multiple times

Despite ongoing research into the pathophysiological role of ferroptosis, numerous studies have shown that gut microbiota can regulate iron balance and oxidative stress through various metabolic processes, influencing ferroptosis in IBD

Notably, the glutathione S-transferase proteins represent an extended family of multifunctional enzymes that are involved in diverse pathways such as oxidative stress response, and detoxification of various substrates 28

When GPX4 is inhibited or depleted, lipid peroxidation spirals out of control, leading to cell death ( - , which imports cystine for glutathione (GSH) synthesis, also plays a crucial role

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