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doi: 10.3390/nu10091203 17 EvansSOKhairuddinPFJamesonMB

NCOA4-Mediated Ferritinophagy Is a pancreatic cancer dependency via maintenance of iron bioavailability for iron-sulfur cluster proteins

The recombinant proteins were extracted with ice-cold lysis buffer containing 20 mM Tris-HCl (pH 7.4), 0.5% Triton X-100, 150 mM NaCl and 2 mM EDTA and then were affinity purified using glutathione Sepharose 4B beads (GE healthcare, Chicago, IL, USA)

It is well established that sleep promotes various cognitive functions, participates in the consolidation of fresh memory traces, and has effects in brain maturation [6]

[DOI] [PMC free article] [PubMed] [Google Scholar] Manzer Z

We therefore used female animals in our study