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Numerous studies have illustrated that the extended exposure to cuprizone induces chronic lesions that show a limited endogenous remyelination capacity (Leo, 2022), prolonged astrogliosis (Hibbits, 2012), and more extensive axonal damage (Lindner, 2009)

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Total RNA was extracted using TRIzol reagent and quantified with NanoDrop 2000

Free radicals weaken the immune system, leading to various diseases and premature aging

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