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GST pulldowns, which we discussed above, are probably the most common form of this type of experiment, but you can perform analogous isolation experiments with other protein tags including his-tags, flag-tags, and strep-tags (Figure 8)

For immunohistochemical stainings, paraffin-embedded tibia sections were incubated overnight with primary antibodies against 8-OHdG (ab10802, Abcam, 1:500), ATF4 (11815, Cell Signaling Technology, 1:20), BrdU (proliferating cells

10.1371/journal.pone.0060733 50 JungM

Programmed cell death is a critical area of research in biology and medicine

One potential downstream gene, CHAC1, was significantly down-regulated in resistant cells (compared with parental ones), and upregulated in GDIL knockdown resistant cells (compared with control) (Fig

The injections are usually subcutaneous (under the skin) or intramuscular (into the muscle), depending on the injury site