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The ex vivo synthesized sequences of FAPCAR were cloned into IVT template plasmid as the ORF region which also carried T7 promoter, modified 5 UTR, kozak sequence, 3 UTR, and a 110 nt-long poly (A) tail 59,60,61

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Side effects are generally minimal and temporary
Populations at a higher risk of vitamin B12 deficiency include people who are vegetarian or vegan, exclusively breastfed infants of vegan women, older adults, those with gastrointestinal disorders or who have had gastrointestinal surgery, and people with an autoimmune condition known as pernicious anemia
The liver stores approximately two to five years' worth of B12, which means deficiency may not become clinically apparent until well after surgery
