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An orthogonal protecting group approach was employed, where all Dab side chain amines were protected as Boc, threonine as tBu, and the amino group of Dab involved in the cyclization was protected as Alloc

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The National Weight Control Registry, which tracks individuals who have successfully maintained significant weight loss, identifies common characteristics including high levels of physical activity, regular breakfast consumption, consistent eating patterns across weekdays and weekends, and frequent self-weighing

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