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FIGURE 2 Dataset construction Positive peptides were curated from B3Pdb, an experimentally validated database of BBB-penetrating peptides (UniProt Consortium, 2023) using BBB-related exclusion keywords (e.g., blood brain barrier, brain, brainpeps, b3pdb, permeation, permeability, transmembrane, transport, transfer, membrane, neuro, and hemolysis), and duplicate sequences were removed from both classes