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The filtered gene-cell matrix were processed using the Seurat package (v.4.3.0) 70 , which included normalization using the NormalizeData function to obtain normalized UMI (unique molecular identifier) count data, scaling, and centering the data using ScaleData , finding the most significant principal components (PCA) using RunPCA and using the ElbowPlot to determine the number of principal components used for clustering

Yale J Biol Med

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