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Maternal immune activation dysregulation of the fetal brain transcriptome and relevance to the pathophysiology of autism spectrum disorder

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Three pairs of starters were used: GSTM1 - sense G1 (GAACTCCCTGAAAAGCTAAAGC) GSTM1 - anti G2 (GTTGGGCTCAAATATACGGTGG) GSTT1 - sense T1 (TTCCTTACTGGTCCTCACATCTC) GSTT1 - anti T2 (TCACCGGATCATGGCCAGCA) Albumin - sense A1 (GCCCTCTGCTAACAAGTCCTAC) Albumin - anti A2 (GCCCTAAAAAGAAAATCGCCAATC) Multiplex PCR proceeded according to the following protocol: DNA initial denaturation at 94 C for 5 min, initial hybridization at 64 C for 2 min, 30 cycles of elongation for 30 s at 72 C, denaturation for 20 s at 94 C, hybridization for 20 s at 64 C, and then final extension for 7 min at 72 C

[DOI] [PubMed] [Google Scholar] 36.Kim J.-S., Wilson J.M., Lee S.-R

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Assay of enzyme activity GPx activities of rhGPx3 mutant were determined using a previously described method 29