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After incubation for 1 h at 37 C, the plates were washed and then incubated with total anti-mouse IgG (Cat

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Certain enteric pathogens possess mechanisms that enable their direct adherence to IECS while others permit the secretion of effector proteins or enterotoxins via membrane localised secretion systems that typically target TJs thereby increasing intestinal permeability by disrupting the intracellular cytoskeleton of IECs (Knig et al

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The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cells