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Innov Food Sci Emerg 12:7378 [Google Scholar] Garcia-Parrilla MC (2008) Antioxidantes en la dieta mediterrnea

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After 15 min agitation at 8 C followed by 15 min centrifugation at 20800 g and 4 C, the supernatant was collected in a 15 ml Falcon tube and protein content was measured using the DC TM Protein Assay (Biorad, Feldkirchen, Germany)