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For the intestinal mechanical barrier hypothesis, gene silencing techniques could be used to specifically knock down key tight junction proteins (e.g., Occludin, ZO-1), or chemical agents could be applied to disrupt barrier integrity, thereby testing whether barrier restoration is a necessary prerequisite for TCM's efficacy

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[DOI] [PMC free article] [PubMed] [Google Scholar] 55.zer A., Demirta H., omu F.M., Erer D., Kl Y., Mardin B., Arslan M., Kk A., Oktar G.L

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The effective concentration in cellular assays is 10 to 100 nM, with loss of efficacy at higher concentrations

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