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2.8 RNA isolation, cDNA synthesis and quantitative Real Time-PCR (qRT-PCR) RNA extraction was performed on whole flies (3 samples per group) using Trizol-chloroform (RNAiso Plus-TaKaRa 99108, Chloroform 99.5% SRL-84155)

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Giardiello FM, Casero RA Jr, Hamilton SR, Hylind LM, Trimbath JD, Geiman DE, Judge KR, Hubbard W, Offerhaus GJA, Yang VW (2004) Prostanoids, ornithine decarboxylase, and polyamines in primary chemoprevention of familial adenomatous polyposis