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PMID: 18073313 Ghigo E, et al

Preparation of a biphasic scaffold for osteochondral tissue engineering

[PMC free article] [PubMed] [Google Scholar] 31.Jiang Y., Zhang T., Kusumanchi P., Han S., Yang Z., Liangpunsakul S

Learn more about vegetarian and vegan sources of vitamin B12

The cell pellet was washed once with 1x PBS supplemented with 100 g/mL cycloheximide before resuspending it in Lysis Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.5% IGEPAL CA-630 (Sigma-Aldrich), protease-inhibitor mix (homemade), 100 g/mL cycloheximide, 2 U/mL SUPERaseIn RNase Inhibitor (Thermo Fisher Scientific))

Kidney Int 73:288299 [DOI] [PubMed] [Google Scholar] Tang D, Zeng T, Wang Y, Cui H, Wu J, Zou B, Tao Z, Zhang L, Garside GB, Tao S (2020) Dietary restriction increases protective gut bacteria to rescue lethal methotrexate-induced intestinal toxicity
